Trichoderma harzianum is a common antagonistic fungus with extensive biological control application value. The culture and propagation method of Trichoderma harzianum is given below:
Medium preparation
Cut potatoes (potatoes) into small pieces and peel them, then add them to distilled water and boil them , then filter out the residue, then add the obtained juice to a culture medium made of sucrose and agar, and mix evenly.
Reproduction and culture
First, use 96% alcohol to extract Trichoderma harzianum under disinfected conditions. Then inoculate it into the prepared culture medium, and place the culture dish in the appropriate temperature (28-30°C) and humidity (40-60%) for 12-18 hours. After it grows, it can be packaged. .
Storage
Put the packaged Trichoderma harzianum in the refrigerator (4℃) for long-term storage.
It should be noted that when cultivating and propagating Trichoderma harzianum, the relevant operating procedures and health and safety requirements should be strictly followed to ensure that the operation process is clean and sanitary, and the culture medium is not contaminated. At the same time, regular inspection and maintenance should be carried out to ensure its quality and breeding effect.
